ABSTRACT
Objective: The resveratrol nanocarrier complex (Res-PAMAM-Ac) was encapsulated by polyamide-amine dendrimer, its stability and safety was investigated. Methods: The blank carriers were synthesized by the divergence method and acetylated. Characterization of the nanocarriers was investigated by 1H-NMR and IR. The properties of carriers were investigated by particle size and zeta potential. Res-PAMAM-Ac was prepared by reverse-phase evaporation method, and the drug loading, encapsulation rate and stability were examined by HPLC. The cytotoxicity of nanocarriers and complex on human lung cancer A549 cells were investigated by MTT method. The biosafety of nanocarriers and complex was inspected by hemolysis test. Results: Res-PAMAM-Ac nanocarrier complex was synthesized successfully. The particle size of Res-PAMAM-Ac was (167.30 ± 21.70) nm, PDI was 0.115 ± 0.006 and the zeta potential was (19.27 ± 0.35) mV. The average drug loading of Res-PAMAM-Ac was (76.99 ± 1.30) mg/g and the encapsulation rate was (29.63 ± 2.7)%. The stability parameter (KE) was less than 0.15, no obvious drug precipitation was observed. Cytotoxicity tests showed that Res-PAMAM-Ac was less toxic to human lung cancer A549 cells when the concentration was below 30 μg/mL. The hemolysis rate of the nanocarriers and complex was less than 5%, which can be considered as biosafety. Conclusion: Res-PAMAM-Ac nanocarrier complex with uniform particle size, high stability, good drug loading and low toxicity was prepared.